배양된 인간 골막기원세포의 조골세포 분화과정에서 골기질 형성정도와 혈관내피세포성장인자 신호와의 상관관계
Correlation between vascular endothelial grwoth factor signaling and mineralization during osteoblastic differentiation of cultured human periosteal-derived cells
박봉욱, 김종렬, 조영철, 성일용, 류영모, 변준호, 하영술, 김덕룡,
소속 상세정보
박봉욱 ( Park Bong-Wook ) - 경상대학교병원 구강악안면외과학교실
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치과대학 구강안면외과학교실
조영철 ( Cho Yeong-Cheol ) - 울산대학교 의과대학 울산대학교병원 구강악안면외과학교실
성일용 ( Sung Iel-Yong ) - 울산대학교 의과대학 울산대학교병원 구강악안면외과학교실
류영모 ( Ryu Young-Mo ) - 경상대학교 의과대학 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 경상대학교 의과대학 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 경상대학교 의과대학 생화학교실
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의과대학 생화학교실
KMID : 0360120070290030197
Abstract
Angiogenesis is a essential part for bone formation and bone fracture healing. Vascular endothelial growth factor (VEGF), one of the most important molecules among many angiogenic factors, is a specific mitogen for vascular endothelial cells. VEGF-mediated angiogenesis is required for bone formation and repair. However, the effect of VEGF on osteoblastic cells during osteogenesis is still controversial. In recent days, substantial progress have been made toward developing tissue-engineered alternatives to autologous bone grafting for maxillofacial bony defects. Periosteum has received considerable interest as a better source of adult stem cells. Periosteum has the advantage of easy harvest and contains various cell types and progenitor cells that are able to differentiate into a several mesenchymal lineages, including bone. Several studies have reported the bone formation potential of periosteal cells, however, the correlation between VEGF signaling and cultured human periosteal cell-derived osteogenesis has not been fully investigated yet. The purpose of this study was to examine the correlation between VEGF signaling and cultured human periosteal-derived cells osteogenesis. Periosteal tissues of 5 x 20 mm were obtained from mandible during surgical extraction of lower impacted third molar from 3 patients. Periostealderived cells were introduced into the cell culture and were subcultured once they reached confluence. After passage 3, the periosteal-derived cells were further cultured for 42 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. We evaluated the alkaline phosphatase (ALP) activity, the expression of Runx2 and VEGF, alizarin red S staining, and the quantification of osteocalcin and VEGF secretion in the periosteal-derived cells.
키워드
Periosteal-derived cell;Osteoblastic differentiation;Mineralization;VEGF signaling
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